<p>(A) Exponentially growing cells were serially diluted (1:10) and each dilution was spotted out onto YEPD plates with or without CPT, phleomycin or MMS. (B) DSB repair by SSA. The analysis was performed as described in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005685#pgen.1005685.g004" target="_blank">Fig 4A</a>. (C) Densitometric analysis of the product band signals. The experiment as in (B) was independently repeated three times and the mean values are represented with error bars denoting s.d. (n = 3). (D) Exponentially growing cells were serially diluted (1:10) and each dilution was spotted out onto YEPD plates with or without CPT, phleomycin or MMS.</p
<p>Diploid <i>sae2</i>, <i>sae2 pch2</i> and <i>sae2 tel1</i> mutants in the <i>NDT80</i> positive b...
<p>H460 cells that were grown on glass slides were transfected with 50 nM of control-siRNA (siCON), ...
<p>A-C. Trypan blue exclusion assays. The G93A mutation was the most susceptible to oxidative stress...
<p>(A) Exponentially growing cells were serially diluted (1:10) and each dilution was spotted out on...
<p>(A) DSB repair by SSA. The analysis was performed as described in <a href="http://www.plosgenetic...
<p>(A) DSB repair by SSA. YEPR exponentially growing cell cultures of YMV45 derivative strains, carr...
<p>(A) Schematic representation of replication-associated DSBs. SSBs accumulate in the genome when c...
<p>(A) Mutation of endonuclease <i>SLX4</i> or DNA repair protein <i>ESC4</i>/<i>RTT107</i> has litt...
<p>(A-D) Exponentially growing cells were serially diluted (1:10) and each dilution was spotted out ...
<p><b>A</b>. Sae2 sumoylation does not affect its protein levels. Sae2-K97R (KR) protein levels are ...
<p><b>(A)</b> SDE2 knockdown leads to increased levels of γH2AX upon DNA damage. HeLa cells transfec...
<p>(A) Spot tests. Ten-fold serial dilutions of strains BY4741 (<i>WT</i>), <i>dun1</i>Δ, <i>sml1</i...
<p>(A) Immunostaining analysis of activated DNA-PKcs in human fibroblasts following the treatment wi...
A. Both mismatches and nonhomologous tails reduce SSA. The frequency of DSB-induced SSA was measured...
<p>(<b>A</b>) Western blot analysis of SLD5 expression in WT and SLD5<sup>+/−</sup> MEFs. β-actin wa...
<p>Diploid <i>sae2</i>, <i>sae2 pch2</i> and <i>sae2 tel1</i> mutants in the <i>NDT80</i> positive b...
<p>H460 cells that were grown on glass slides were transfected with 50 nM of control-siRNA (siCON), ...
<p>A-C. Trypan blue exclusion assays. The G93A mutation was the most susceptible to oxidative stress...
<p>(A) Exponentially growing cells were serially diluted (1:10) and each dilution was spotted out on...
<p>(A) DSB repair by SSA. The analysis was performed as described in <a href="http://www.plosgenetic...
<p>(A) DSB repair by SSA. YEPR exponentially growing cell cultures of YMV45 derivative strains, carr...
<p>(A) Schematic representation of replication-associated DSBs. SSBs accumulate in the genome when c...
<p>(A) Mutation of endonuclease <i>SLX4</i> or DNA repair protein <i>ESC4</i>/<i>RTT107</i> has litt...
<p>(A-D) Exponentially growing cells were serially diluted (1:10) and each dilution was spotted out ...
<p><b>A</b>. Sae2 sumoylation does not affect its protein levels. Sae2-K97R (KR) protein levels are ...
<p><b>(A)</b> SDE2 knockdown leads to increased levels of γH2AX upon DNA damage. HeLa cells transfec...
<p>(A) Spot tests. Ten-fold serial dilutions of strains BY4741 (<i>WT</i>), <i>dun1</i>Δ, <i>sml1</i...
<p>(A) Immunostaining analysis of activated DNA-PKcs in human fibroblasts following the treatment wi...
A. Both mismatches and nonhomologous tails reduce SSA. The frequency of DSB-induced SSA was measured...
<p>(<b>A</b>) Western blot analysis of SLD5 expression in WT and SLD5<sup>+/−</sup> MEFs. β-actin wa...
<p>Diploid <i>sae2</i>, <i>sae2 pch2</i> and <i>sae2 tel1</i> mutants in the <i>NDT80</i> positive b...
<p>H460 cells that were grown on glass slides were transfected with 50 nM of control-siRNA (siCON), ...
<p>A-C. Trypan blue exclusion assays. The G93A mutation was the most susceptible to oxidative stress...