Type I Collagen degradation assay.

<p>Collagen degradation measured in culture supernatants from canine endothelial (A) and smooth muscle cells (B) untreated or treated with 10 μg/ml of PLG, 1 μg/ml of rDiACT/rDiFBAL, 1 μg/ml of rDiACT/rDiFBAL + 10 μg/ml of PLG or with 1 μg/ml of rDiACT/rDiFBAL + 10 μg/ml of PLG + 50 mM of the εACA. The results were expressed as percentage of the Type I Collagen concentration in the culture supernatant from negative control cells (100%). Each point is the mean ± SD from three independent experiments. The asterisk (*) designates significant (p<0.05) differences between rDiACT or rDiFBAL + PLG treatment and control groups.