MMP-2 and 9 levels assay.

<p>Representative zymography of MMP-2 (solid bars) and MMP-9 (hatched bars) levels in the culture supernatants from canine endothelial (A) and smooth muscle cells (B) untreated or treated with 10 μg/ml of PLG, 1 μg/ml of rDiACT/rDiFBAL, 1 μg/ml of rDiACT/rDiFBAL + 10 μg/ml of PLG or with 1 μg/ml of rDiACT/rDiFBAL + 10 μg/ml of PLG + 50 mM of the εACA. Note the gelatinolytic bands associated with MMP-2 (72 KDa) and MMP-9 (92 KDa) levels, as well as with the MMP-9 activated form (marked with a white asterisk at 82KDa. The results were expressed as percentage of the MMPs levels in the culture supernatant from negative control cells (100%). Data are shown as representative images or means ± SD from three independent experiments. The asterisk (*) designates significant (p<0.05) differences between rDiACT or rDiFBAL treatment and control groups.