MMPs of CDLs in compliant, standard and stiff conditions visualized by gelatin zymography.

<p>(A) Zymogram showing pro-enzyme and active MMP band profile of CDLs in the three mechanical states. (B) Zymogram comparing the band profiles of CDLs, compared with human cell line showing MMP-2 and MMP-9 activity; this zymogram was included because of its very good band separation. (C) Optical density of MMP activity in CDLs in the three mechanical states. (D) Comparative densitometric analysis of scanned gels of CDLs in the different mechanical states with and without 50 µM galardin. Data are expressed as means ± SD. The asterisk (*) represents statistically significant difference P<0.05. MMPs were detected in more than six animals for each of the three mechanical states. (E) Zymogram comparing standard CDLs with and without galardin treatment.