Western blot analysis of collagen type I.

<p>(A) Representative immunoblot showing collagen type I levels (upper) in A/J and 129P3/J mice after treatment with 0, 10 or 50 ppmF in drinking water for 8 weeks. The level of β-tubulin was used as control of sample loading (lower). (B) Values are mean ± SD of arbitrary values of four independent experiments quantified by densitometry analysis using the Image J Software (NIH Image). Arbitrary values from control A/J were standardized as 1. ^**Represents significant differences between strains for each treatment (<i>p</i><0.01). No statistical differences were found for each strain after receiving F.