Efficacy of the <i>VPS13A</i> knockdown shRNAs in lentivirus infected cells.

<p>(A) Western blot using anti-chorein antibodies to determine the level chorein expression in control and lentivirus-infected knockdown cells. The levels of GAPDH were used as a loading control for each lysate. For chorein and GAPDH detection, 100μg and 50μg of total lysates were loaded, respectively. (B) Quantitation of PtdIns-phosphate sensor localization to the plasma membrane in in control and lentivirus-infected knockdown cells. Two independent experiments were performed for each reporter. At least 50 cells scored in each experiment. Error bars indicate one standard deviation. Asterisks indicate a significant reduction of PtdIns(4)P sensor localization to the plasma membrane in cells expressing knockdown shRNA #3 or #5 relative to those expressing the scrambled shRNA (p<0.0001, Chi square test).