shRNAs silencing transient expression of viral genes in 293T cells.

Stable knock down of XMRV in 22Rv1 cells.

Kristin Stieler (149145)
Udo Schumacher (149148)
Andrea Kristina Horst (149150)
Nicole Fischer (62348)

July 2012

(A) Schematic representation of XMRV LTR region and 5′ UTR of Gag. Localization of shRNAs ...

Viral titers in FLNA knockdown cells.

Fyza Y. Shaikh (151284)
Thomas J. Utley (151287)
Ryan E. Craven (151291)
Meredith C. Rogers (151296)
Lynne A. Lapierre (114938)
James R. Goldenring (114947)
James E. Crowe Jr (151303)

July 2012

HEp-2 cells were transduced with a lentivirus encoding an shRNA directed against the indicated pr...

Lentiviral LVTPT system allows for inducible cDNA and shRNA expression in stable cell lines and primary neurons.

David C. Bersten (510511)
Adrienne E. Sullivan (701583)
Dian Li (701584)
Veronica Bhakti (701585)
Stephen J. Bent (701586)
Murray L. Whitelaw (215366)

March 2015

(a) Vector diagram of constitutively expressed (Tet-On 3G and eGFP) and Doxycycline-inducible (ds...

shRNAs targeting viral gene and inhibiting replication of FMDV in BHK-21 cells.

Hongmei Wang (190581)
Jianming Wu (312135)
Xiao Liu (65413)
Hongbin He (312136)
Fangrong Ding (270039)
Hongjun Yang (138270)
Lei Cheng (171055)
Wenhao Liu (312138)
Jifeng Zhong (312141)
Yunping Dai (147821)
Guangpeng Li (312144)
Chengqiang He (312146)
Li Yu (81651)
Jianbin Li (312148)

February 2013

BHK-21 cells stable expressing shRNA were cultured in 12-well plates in four replicates, each wel...

Validation of shRNA clones for gene silencing in 293FT cells

Wang, Wen

January 2008

...The goal of the project was to establish knock down of mRNA in human mesenchymal stem cells. Sinc...

AHDC1 knockdown of A673 cells by lentivirus expressing shRNA.

Takao Kitagawa (618877)
Daiki Kobayashi (414250)
Byron Baron (637446)
Hajime Okita (240986)
Tatsuo Miyamoto (12225655)
Rie Takai (13905663)
Durga Paudel (12635367)
Tohru Ohta (12917207)
Yoichi Asaoka (564124)
Masayuki Tokunaga (13905666)
Koji Nakagawa (424076)
Makoto Furutani-Seiki (24192)
Norie Araki (111307)
Yasuhiro Kuramitsu (618885)
Masanobu Kobayashi (424082)

October 2022

Lentivirus expressing shRNA was transduced to A673 cells for 3 d. Each protein was detected by its r...

Knockdown of SMC1 with shRNA blocks viral genome amplification.

Kavi Mehta (721587)
Vignesh Gunasekharan (721588)
Ayano Satsuka (721589)
Laimonis A. Laimins (258973)

April 2015

A). CIN612 were infected with lentiviruses encoding shRNAs to SMC1 and after 48 hours total cell ...

Packaging shRNA retroviruses

Chang, K.
Marran, K.
Valentine, A.
Hannon, G. J.

January 2013

To silence a mammalian gene by RNAi using an encoded trigger, a short-hairpin RNA (shRNA) is integra...

Lentivirus-mediated miR-223 over-expression in HeLa cells.

Cheng You Jia (340599)
Hui Hui Li (340602)
Xu Chao Zhu (340603)
Yi Wei Dong (340604)
Da Fu (114238)
Qian Lei Zhao (340606)
Wei Wu (27708)
Xing Zhong Wu (340608)

February 2013

(A) Lentivirus vector pLL3.7-miR-223 was packaged in HEK-293T cells which was less 20 generations...

Lentivirus titration and MOI-directed shRNA transduction.

Christiane Schaefer (4811433)
Nikhil Mallela (4811439)
Jochen Seggewiß (830782)
Birgit Lechtape (4811427)
Heymut Omran (4811436)
Uta Dirksen (282783)
Eberhard Korsching (387671)
Jenny Potratz (4811430)

January 2018

(A) Titration curves of non-silencing and LaminA/C shRNA viruses. A673 cells were transduced with...

AHDC1 knockdown shows reduced growth of Seki and NCR-EW2 by lentivirus expressing shRNA.

Takao Kitagawa (618877)
Daiki Kobayashi (414250)
Byron Baron (637446)
Hajime Okita (240986)
Tatsuo Miyamoto (12225655)
Rie Takai (13905663)
Durga Paudel (12635367)
Tohru Ohta (12917207)
Yoichi Asaoka (564124)
Masayuki Tokunaga (13905666)
Koji Nakagawa (424076)
Makoto Furutani-Seiki (24192)
Norie Araki (111307)
Yasuhiro Kuramitsu (618885)
Masanobu Kobayashi (424082)

October 2022

(A) Lentivirus expressing shRNA was transduced to Seki or NCR-EW2 cells for 3 d. 1 × 103 Cells were ...

Lentivirus-mediated shRNA targeting CXCR7 effectively silenced CXCR7 in MOLT4 and Jurkat cells.

Rita de Cássia Carvalho Melo (517828)
Ana Leda Longhini (517829)
Carolina Louzão Bigarella (517830)
Mariana Ozello Baratti (517831)
Fabiola Traina (143689)
Patrícia Favaro (517832)
Paula de Melo Campos (517833)
Sara Teresinha Olalla Saad (517834)

January 2014

A) Quantitative expression of CXCR7 mRNA in cells relative to the shControl cells. mRNA ex...

Design and validation of 14-3-3ζ shRNA constructs

Heidi M. B. Lesscher (322409)
Julia M. Houthuijzen (322410)
Marian J. Groot Koerkamp (198314)
Frank C. P. Holstege (117137)
Louk J. M. J. Vanderschuren (318956)

February 2013

A 14-3-3ζ specific shRNA sequences (1222 and 1854 bp) were cloned into pLentiLox3.7...

shRNA-expressing plasmids mediate the allele-specific silencing of endogenous wild-type ataxin-3 in transiently transfected human 293T cells.

Sandro Alves (376415)
Isabel Nascimento-Ferreira (275747)
Gwennaëlle Auregan (376416)
Raymonde Hassig (376417)
Noëlle Dufour (5653876)
Emmanuel Brouillet (46542)
Maria C. Pedroso de Lima (376418)
Philippe Hantraye (46540)
Luís Pereira de Almeida (221526)
Nicole Déglon (46541)

February 2013

A) Western blot of human 293T cells transfected with different shRNA (shAtaxWT, shAtaxMUT and shG...

Knockdown of USP9X in U87 GB cells.

Jesse L. Cox (139259)
Phillip J. Wilder (29780)
Joshua M. Gilmore (259014)
Erin L. Wuebben (173367)
Michael P. Washburn (127462)
Angie Rizzino (29782)

May 2013

(A) Western blot analysis to verify the knockdown of USP9X in U87 GB cells following infection wi...

Stable knock down of XMRV in 22Rv1 cells.

Kristin Stieler (149145)
Udo Schumacher (149148)
Andrea Kristina Horst (149150)
Nicole Fischer (62348)

July 2012

(A) Schematic representation of XMRV LTR region and 5′ UTR of Gag. Localization of shRNAs ...

Viral titers in FLNA knockdown cells.

Fyza Y. Shaikh (151284)
Thomas J. Utley (151287)
Ryan E. Craven (151291)
Meredith C. Rogers (151296)
Lynne A. Lapierre (114938)
James R. Goldenring (114947)
James E. Crowe Jr (151303)

July 2012

HEp-2 cells were transduced with a lentivirus encoding an shRNA directed against the indicated pr...

Lentiviral LVTPT system allows for inducible cDNA and shRNA expression in stable cell lines and primary neurons.

David C. Bersten (510511)
Adrienne E. Sullivan (701583)
Dian Li (701584)
Veronica Bhakti (701585)
Stephen J. Bent (701586)
Murray L. Whitelaw (215366)

March 2015

(a) Vector diagram of constitutively expressed (Tet-On 3G and eGFP) and Doxycycline-inducible (ds...

shRNAs targeting viral gene and inhibiting replication of FMDV in BHK-21 cells.

Hongmei Wang (190581)
Jianming Wu (312135)
Xiao Liu (65413)
Hongbin He (312136)
Fangrong Ding (270039)
Hongjun Yang (138270)
Lei Cheng (171055)
Wenhao Liu (312138)
Jifeng Zhong (312141)
Yunping Dai (147821)
Guangpeng Li (312144)
Chengqiang He (312146)
Li Yu (81651)
Jianbin Li (312148)

February 2013

BHK-21 cells stable expressing shRNA were cultured in 12-well plates in four replicates, each wel...

Validation of shRNA clones for gene silencing in 293FT cells

Wang, Wen

January 2008

...The goal of the project was to establish knock down of mRNA in human mesenchymal stem cells. Sinc...

AHDC1 knockdown of A673 cells by lentivirus expressing shRNA.

Takao Kitagawa (618877)
Daiki Kobayashi (414250)
Byron Baron (637446)
Hajime Okita (240986)
Tatsuo Miyamoto (12225655)
Rie Takai (13905663)
Durga Paudel (12635367)
Tohru Ohta (12917207)
Yoichi Asaoka (564124)
Masayuki Tokunaga (13905666)
Koji Nakagawa (424076)
Makoto Furutani-Seiki (24192)
Norie Araki (111307)
Yasuhiro Kuramitsu (618885)
Masanobu Kobayashi (424082)

October 2022

Lentivirus expressing shRNA was transduced to A673 cells for 3 d. Each protein was detected by its r...

Knockdown of SMC1 with shRNA blocks viral genome amplification.

Kavi Mehta (721587)
Vignesh Gunasekharan (721588)
Ayano Satsuka (721589)
Laimonis A. Laimins (258973)

April 2015

A). CIN612 were infected with lentiviruses encoding shRNAs to SMC1 and after 48 hours total cell ...

Packaging shRNA retroviruses

Chang, K.
Marran, K.
Valentine, A.
Hannon, G. J.

January 2013

To silence a mammalian gene by RNAi using an encoded trigger, a short-hairpin RNA (shRNA) is integra...

Lentivirus-mediated miR-223 over-expression in HeLa cells.

Cheng You Jia (340599)
Hui Hui Li (340602)
Xu Chao Zhu (340603)
Yi Wei Dong (340604)
Da Fu (114238)
Qian Lei Zhao (340606)
Wei Wu (27708)
Xing Zhong Wu (340608)

February 2013

(A) Lentivirus vector pLL3.7-miR-223 was packaged in HEK-293T cells which was less 20 generations...

Lentivirus titration and MOI-directed shRNA transduction.

Christiane Schaefer (4811433)
Nikhil Mallela (4811439)
Jochen Seggewiß (830782)
Birgit Lechtape (4811427)
Heymut Omran (4811436)
Uta Dirksen (282783)
Eberhard Korsching (387671)
Jenny Potratz (4811430)

January 2018

(A) Titration curves of non-silencing and LaminA/C shRNA viruses. A673 cells were transduced with...

AHDC1 knockdown shows reduced growth of Seki and NCR-EW2 by lentivirus expressing shRNA.

Takao Kitagawa (618877)
Daiki Kobayashi (414250)
Byron Baron (637446)
Hajime Okita (240986)
Tatsuo Miyamoto (12225655)
Rie Takai (13905663)
Durga Paudel (12635367)
Tohru Ohta (12917207)
Yoichi Asaoka (564124)
Masayuki Tokunaga (13905666)
Koji Nakagawa (424076)
Makoto Furutani-Seiki (24192)
Norie Araki (111307)
Yasuhiro Kuramitsu (618885)
Masanobu Kobayashi (424082)

October 2022

(A) Lentivirus expressing shRNA was transduced to Seki or NCR-EW2 cells for 3 d. 1 × 103 Cells were ...

Lentivirus-mediated shRNA targeting CXCR7 effectively silenced CXCR7 in MOLT4 and Jurkat cells.

Rita de Cássia Carvalho Melo (517828)
Ana Leda Longhini (517829)
Carolina Louzão Bigarella (517830)
Mariana Ozello Baratti (517831)
Fabiola Traina (143689)
Patrícia Favaro (517832)
Paula de Melo Campos (517833)
Sara Teresinha Olalla Saad (517834)

January 2014

A) Quantitative expression of CXCR7 mRNA in cells relative to the shControl cells. mRNA ex...

Design and validation of 14-3-3ζ shRNA constructs

Heidi M. B. Lesscher (322409)
Julia M. Houthuijzen (322410)
Marian J. Groot Koerkamp (198314)
Frank C. P. Holstege (117137)
Louk J. M. J. Vanderschuren (318956)

February 2013

A 14-3-3ζ specific shRNA sequences (1222 and 1854 bp) were cloned into pLentiLox3.7...

shRNA-expressing plasmids mediate the allele-specific silencing of endogenous wild-type ataxin-3 in transiently transfected human 293T cells.

Sandro Alves (376415)
Isabel Nascimento-Ferreira (275747)
Gwennaëlle Auregan (376416)
Raymonde Hassig (376417)
Noëlle Dufour (5653876)
Emmanuel Brouillet (46542)
Maria C. Pedroso de Lima (376418)
Philippe Hantraye (46540)
Luís Pereira de Almeida (221526)
Nicole Déglon (46541)

February 2013

A) Western blot of human 293T cells transfected with different shRNA (shAtaxWT, shAtaxMUT and shG...

Knockdown of USP9X in U87 GB cells.

Jesse L. Cox (139259)
Phillip J. Wilder (29780)
Joshua M. Gilmore (259014)
Erin L. Wuebben (173367)
Michael P. Washburn (127462)
Angie Rizzino (29782)

May 2013

(A) Western blot analysis to verify the knockdown of USP9X in U87 GB cells following infection wi...

Stable knock down of XMRV in 22Rv1 cells.

Kristin Stieler (149145)
Udo Schumacher (149148)
Andrea Kristina Horst (149150)
Nicole Fischer (62348)

July 2012

(A) Schematic representation of XMRV LTR region and 5′ UTR of Gag. Localization of shRNAs ...

Viral titers in FLNA knockdown cells.

Fyza Y. Shaikh (151284)
Thomas J. Utley (151287)
Ryan E. Craven (151291)
Meredith C. Rogers (151296)
Lynne A. Lapierre (114938)
James R. Goldenring (114947)
James E. Crowe Jr (151303)

July 2012

HEp-2 cells were transduced with a lentivirus encoding an shRNA directed against the indicated pr...

Lentiviral LVTPT system allows for inducible cDNA and shRNA expression in stable cell lines and primary neurons.

David C. Bersten (510511)
Adrienne E. Sullivan (701583)
Dian Li (701584)
Veronica Bhakti (701585)
Stephen J. Bent (701586)
Murray L. Whitelaw (215366)

March 2015

(a) Vector diagram of constitutively expressed (Tet-On 3G and eGFP) and Doxycycline-inducible (ds...

shRNAs silencing transient expression of viral genes in 293T cells.

Abstract

Extracted data

shRNAs silencing transient expression of viral genes in 293T cells.

Abstract

Extracted data

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