Influence of USP9x knockdown on the distributions of marker proteins of ER, Golgi and lysosomes.

<p>(A) HEK293 cells infected by the lentivirus expressing pLVX-shRNA1 (non-targeting), USP9x-shRNA2, or USP9x-shRNA3 were co-stained with anti-USP9x (1 µg/ml) and anti-β-GalT1 (1∶2500) (the top panels) or co-stained with anti-β-GalT1 (1∶2500) and anti-TGN46 (1∶2500) (the bottom panels). (B) Percentage of cells with condensed or dispersed TGN46 staining. The values on the bar graph represented the average from three independent experiments with more than 500 cells were counted in each experiment (error bars: standard deviation). (C–E) The same set of HEK293 cells were stained with anti-Calreticulin (1∶1000; C), p115 (1∶600; D) or Lamp1 (1∶3000; E), respectively. Scale bar: 20 µm.