Knockdown of STAT-5 suppresses ATM DNA damage responses.

<p>HPV31-positive CIN612 cells were transduced as described in legend to <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003295#ppat-1003295-g003" target="_blank">Figure 3</a>. A) Western blot analysis of p-ATM, ATM, p-CHK2, CHK2, involucrin and GAPDH protein levels in uninfected and shRNA lentivirus infected CIN612 cells upon differentiation in high-calcium media for indicated times. B) Western blot analysis of p-CHK2, CHK2, STAT-5α, STAT-5β, and GAPDH protein levels in shRNA control and shRNA lentivirus infected CIN612 cells upon differentiation in high-calcium media for indicated times. C) Western blot analysis of BRCA-1. BRCA-2, SMC-1, p-SMC-1, RAD51 and GAPDH protein at total or phosphorylation levels in uninfected and shRNA lentivirus infected CIN612 cells upon differentiation for indicated times. The quantification of the band intensities is shown as bar graph figures in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003295#ppat.1003295.s001" target="_blank">Figure S1</a>. All results are representative of observations from 3 independent experiments.