Immunization of C57BL/6 and BALB/cJ mice with LAGAP elicits antibodies against both sporozoites and BS parasites.

<p>Serum from BALB/cJ and C57BL/6 mice (n = 10 mice per group, over two independent immunizations) immunized with 3 x 50,000 <i>Pyfabb/f-</i> sporozoites was collected 2 weeks after the final immunization and used in ELISA to detect total IgG against CSP (A) and blood stage lysate (B). C57BL/6 and BALB/cJ mice produce antibodies against both CSP and BS proteins with higher anti-BS titers in C57BL/6 mice. Comparisons were performed using one-way ANOVA with Tukey post-hoc analysis with significance indicated by: *0.05≥p>0.01; **0.01≥p>0.001; ***0.001≥p>0.0001; ****p≤0.0001; non-significant (NS) p≥0.05.