Novel antigens combined with CSP provide greater protection than CSP alone.

<p>(A) Each C57Bl/6 mouse (circles) was immunized with a combination of <i>Py</i>CSP DNA (2.5 μg) with novel antigen DNA (2.5 μg) by GG, using the schedule described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0159449#pone.0159449.g002" target="_blank">Fig 2</a>. (B) Each BALB/c mouse (triangles) was immunized with a combination of <i>Pb</i>CSP DNA (10 μg) with novel antigen DNA (10 μg) by EP, using the same schedule used for the GG immunizations. Data were compared to the negative control group immunized with a combination of CSP and EV tested in the same immunization study. Significant reduction in LS parasite burden was determined by Kruskal-Wallis test followed by Mann-Whitney test and p<0.05 was considered as significant. Green box indicates p<0.05 and red box indicates p>0.05. A complete statistical analysis is provided in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0159449#pone.0159449.s011" target="_blank">S6 Table</a>.