Generation and characterization of B7-H3 KO mice.
- Liqun Luo (1951)
- Gefeng Zhu (258276)
- Haiying Xu (258277)
- Sheng Yao (212684)
- Gang Zhou (233585)
- Yuwen Zhu (753461)
- Koji Tamada (202066)
- Lanqing Huang (224078)
- Andrew D. Flies (753462)
- Megan Broadwater (753463)
- William Ruff (753464)
- Jan M. A. van Deursen (753465)
- Ignacio Melero (81908)
- Zhou Zhu (46647)
- Lieping Chen (258282)
Publication date
June 2015
DOIAbstract
<p>(A) Mapping of the B7-H3 genomic locus, targeting vector, and the replaced allele. (B) Southern blot analysis of SpeI-digested DNA from targeted embryonic stem (ES) cell clones. The wild-type allele generated a 9.6-kb fragment, and the targeted allele yielded an 8.0-kb fragment. (C) PCR identification of genomic DNA isolated from tails of wild type (+/+), heterozygous (+/−) or homozygous (−/−) B7-H3 mutant mice. The wild type allele generated an 894-bp fragment and the targeted allele generated a 632-bp fragment. (D) Examination of B7-H3 mRNA by RT-PCR. The cDNA was generated from the spleens of wild type (+/+), heterozygous (+/−) or homozygous (−/−) B7-H3 mutant mice using primers specific for B7-H3 or β-actin (control).</p
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