Generation of a null <i>Khdrbs3</i> allele.

<p>(A) Northern analysis of different adult mouse tissues to detect expression of <i>Khdrbs3</i> (upper panel) and small subunit rRNA (lower panel). (B) The genomic structure of the <i>Khdrbs3</i> alleles from wild type, floxed, and null mice mice were monitored using Southern blotting and the probe indicated in parts C–D. The Southern blot demonstrates that the cross with a PGK-Cre mouse successfully removed exon 2 from the genomic DNA. (C) Genomic structure of the <i>Khdrbs3^LoxP</i> conditional allele in which exon 2 of the <i>Khdrbs3</i> gene is flanked by <i>Lox</i>P sites. (D) Genomic structure of the null (<i>Khdrbs3⁻</i>) allele from which exon 2 has been deleted by Cre-mediated recombination. (E) Multiplex RT-PCR analysis of <i>Khdrbs3</i> and <i>Hprt</i> mRNA levels in different mouse tissues. The size markers are shown in nucleotides. (F) Western blot analysis of Sam68 and T-STAR protein levels in the testes of wild type and <i>Khdrbs3</i> null mice using an antibody that recognizes T-STAR and Sam68. The position of the size markers are shown in KDa.