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Genotyping of Blk<sup>-/-</sup> mice, related to Fig 2.

Wei-Wei Li (276638)
Xu-Xu Fan (17271368)
Zi-Xiang Zhu (17271371)
Xue-Jing Cao (17271374)
Zhao-Yu Zhu (17271377)
Dan-Shi Pei (17271380)
Yi-Zhuo Wang (9724460)
Ji-Yan Zhang (551912)
Yan-Yi Wang (3930020)
Hai-Xue Zheng (544167)

Publication date

October 2023

DOI

10.1371/journal.ppat.1011742.s003

Abstract

(A)Blk gene targeting strategy. The exon 3–9 and part of intron of Blk gene were deleted by CRISPR/Cas9 method. (B) Genotyping of Blk-/- mice. PCR analysis of genomic DNA to identify wild-type, heterogenous and Blk-/- mice. (TIF)</p

Extracted data

Related items

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<p>(<b>A</b>) The strategy for the generation of a targeted <i>Mbd5</i> allele. Numbered black boxes...

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<div><p>a.The representative gel electropherogram of the genotyping result by BglII digestion of the...

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<p><b>A.</b> Schematic diagram of the strategy used to target the mouse <i>Sdhd</i> locus. The struc...

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<p><i>A. Targeting strategy for generating MLF1-IP KO mice</i> The red square on the 5′ side of the ...

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Tianzhu Chao (6375851)
Zhuangzhuang Liu (6375854)
Yu Zhang (12946)
Lichen Zhang (5431988)
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Le He (577443)
Yanrong Gu (6375857)
Zhijun Chen (254277)
Qianqian Zheng (4811130)
Lijin Shi (6375860)
Wenping Zheng (6375863)
Xinhui Qi (6375866)
Eryan Kong (6375869)
Zhongjian Zhang (300392)
Toby Lawrence (151657)
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February 2019

It is a tempting goal to identify causative genes underlying phenotypic differences among inbred str...

Targeted exogenous gene(s) integration at mouse MYH9 intron 2 site.

Tanbin Liu (4849807)
Yi Hu (58993)
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Yang Zhan (157356)
Lingchen Yang (4849810)
Wei Liu (20030)
Naidong Wang (4725801)
Yalan Li (1696234)
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Chengyu Liu (424209)
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Robert S. Adelstein (59194)
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February 2018

<p>(a). Schematic demonstrating the strategy for targeted integration of exogenous gene(s), wild typ...

Targeted disruption of <i>Mbd5</i> in mice.

Yarui Du (127339)
Bo Liu (127343)
Fan Guo (65416)
Guifang Xu (127349)
Yuqiang Ding (127352)
Yong Liu (6908)
Xin Sun (127355)
Guoliang Xu (67200)

October 2012

<p>(<b>A</b>) The strategy for the generation of a targeted <i>Mbd5</i> allele. Numbered black boxes...

Table_2_Precise and Rapid Validation of Candidate Gene by Allele Specific Knockout With CRISPR/Cas9 in Wild Mice.XLSX

Tianzhu Chao (6375851)
Zhuangzhuang Liu (6375854)
Yu Zhang (12946)
Lichen Zhang (5431988)
Rong Huang (298496)
Le He (577443)
Yanrong Gu (6375857)
Zhijun Chen (254277)
Qianqian Zheng (4811130)
Lijin Shi (6375860)
Wenping Zheng (6375863)
Xinhui Qi (6375866)
Eryan Kong (6375869)
Zhongjian Zhang (300392)
Toby Lawrence (151657)
Yinming Liang (270857)
Liaoxun Lu (179293)

February 2019

It is a tempting goal to identify causative genes underlying phenotypic differences among inbred str...

Inactivation of the tyrosinase locus in C57BL/6NTac ES cells and mice.

Branko Zevnik (257460)
Nathalie C. Uyttersprot (530957)
Ana V. Perez (530958)
Gerald W. M. Bothe (530959)
Heidrun Kern (27114)
Gunther Kauselmann (257458)

March 2014

<p>(A) Gene targeting scheme for the tyrosinase locus. A point mutation was introduced in exon 1 (C1...

Generation and characterization of B7-H3 KO mice.

Liqun Luo (1951)
Gefeng Zhu (258276)
Haiying Xu (258277)
Sheng Yao (212684)
Gang Zhou (233585)
Yuwen Zhu (753461)
Koji Tamada (202066)
Lanqing Huang (224078)
Andrew D. Flies (753462)
Megan Broadwater (753463)
William Ruff (753464)
Jan M. A. van Deursen (753465)
Ignacio Melero (81908)
Zhou Zhu (46647)
Lieping Chen (258282)

June 2015

<p>(A) Mapping of the B7-H3 genomic locus, targeting vector, and the replaced allele. (B) Southern b...

Gene-targeting strategy and genotype analysis.

Usha P. Andley (354477)
Paul D. Hamilton (354478)
Nathan Ravi (354479)
Conrad C. Weihl (354480)

February 2013

<p><i>A)</i> Diagram illustrating the gene-targeting strategy used to produce αB-R120G gene knock-in...

Targeting of the mouse <i>Neu3</i> gene.

Kazunori Yamaguchi (151848)
Kazuhiro Shiozaki (151850)
Setsuko Moriya (151852)
Koichi Koseki (151854)
Tadashi Wada (151856)
Hiroo Tateno (151861)
Ikuro Sato (151864)
Masahide Asano (151869)
Yoichiro Iwakura (23402)
Taeko Miyagi (151874)

July 2012

<p>A. Targeting strategy. Exons and introns are indicated by boxes and lines, respectively. Open box...

LacZ cassette into the VEGFR-3 locus (11). The 3-kb

L. Jussila
J. Taipale
K. Alitalo
Unpublished Data

January 2016

Kpn I—Not I and Bam HI—Kpn I fragments from 129SV mouse genomic DNA library (lFIX II; Stratagene) we...

Generation of TANK1-deficient mice by gene targeting.

Y. Jeffrey Chiang (121063)
Susan J. Hsiao (377250)
Dena Yver (377251)
Samuel W. Cushman (251495)
Lino Tessarollo (45944)
Susan Smith (377252)
Richard J. Hodes (121071)

February 2013

<p>(A) Gene targeting strategy and restriction map of TANK1 gene. Filled boxes indicate exons; label...

Germline transmission of the targeting modification.

Bin Shen (143992)
Xin Zhang (35492)
Yinan Du (225021)
Jianying Wang (475432)
Jun Gong (475433)
Xiaodong Zhang (183379)
Peri H. Tate (142031)
Hongliang Li (122229)
Xingxu Huang (39402)
Wensheng Zhang (41833)

October 2013

<div><p>a.The representative gel electropherogram of the genotyping result by BglII digestion of the...

Targeted exogenous gene(s) integration into the mouse MYH9 gene exon 2 site.

Tanbin Liu (4849807)
Yi Hu (58993)
Shiyin Guo (4849813)
Lei Tan (211429)
Yang Zhan (157356)
Lingchen Yang (4849810)
Wei Liu (20030)
Naidong Wang (4725801)
Yalan Li (1696234)
Yingfan Zhang (508974)
Chengyu Liu (424209)
Yi Yang (116183)
Robert S. Adelstein (59194)
Aibing Wang (4318408)

February 2018

<p>(a). Schematic demonstrating the strategy for targeted integration of exogenous gene(s), wild typ...

Table_3_Precise and Rapid Validation of Candidate Gene by Allele Specific Knockout With CRISPR/Cas9 in Wild Mice.xlsx

Tianzhu Chao (6375851)
Zhuangzhuang Liu (6375854)
Yu Zhang (12946)
Lichen Zhang (5431988)
Rong Huang (298496)
Le He (577443)
Yanrong Gu (6375857)
Zhijun Chen (254277)
Qianqian Zheng (4811130)
Lijin Shi (6375860)
Wenping Zheng (6375863)
Xinhui Qi (6375866)
Eryan Kong (6375869)
Zhongjian Zhang (300392)
Toby Lawrence (151657)
Yinming Liang (270857)
Liaoxun Lu (179293)

February 2019

It is a tempting goal to identify causative genes underlying phenotypic differences among inbred str...

Generation and analysis of <i>Sdhd</i>-deficient mice.

Jean-Pierre Bayley (13430)
Ivonne van Minderhout (369146)
Pancras C. W. Hogendoorn (123300)
Cees J. Cornelisse (369147)
Annemieke van der Wal (195948)
Frans A. Prins (369148)
Luc Teppema (369149)
Albert Dahan (282631)
Peter Devilee (13431)
Peter E. M. Taschner (369150)

February 2013

<p><b>A.</b> Schematic diagram of the strategy used to target the mouse <i>Sdhd</i> locus. The struc...

Generation of MLF1-IP KO mice.

Xuehai Wang (305003)
Martin Marcinkiewicz (305004)
Yaned Gatain (415776)
Maxime Bouchard (216093)
Jianning Mao (415777)
Michel Tremblay (415778)
Noriko Uetani (367750)
Silva Hanissian (415779)
Shijie Qi (305005)
Jiangping Wu (305008)
Hongyu Luo (305007)

May 2013

<p><i>A. Targeting strategy for generating MLF1-IP KO mice</i> The red square on the 5′ side of the ...

Presentation_1_Precise and Rapid Validation of Candidate Gene by Allele Specific Knockout With CRISPR/Cas9 in Wild Mice.PPTX

Tianzhu Chao (6375851)
Zhuangzhuang Liu (6375854)
Yu Zhang (12946)
Lichen Zhang (5431988)
Rong Huang (298496)
Le He (577443)
Yanrong Gu (6375857)
Zhijun Chen (254277)
Qianqian Zheng (4811130)
Lijin Shi (6375860)
Wenping Zheng (6375863)
Xinhui Qi (6375866)
Eryan Kong (6375869)
Zhongjian Zhang (300392)
Toby Lawrence (151657)
Yinming Liang (270857)
Liaoxun Lu (179293)

February 2019

It is a tempting goal to identify causative genes underlying phenotypic differences among inbred str...

Targeted exogenous gene(s) integration at mouse MYH9 intron 2 site.

Tanbin Liu (4849807)
Yi Hu (58993)
Shiyin Guo (4849813)
Lei Tan (211429)
Yang Zhan (157356)
Lingchen Yang (4849810)
Wei Liu (20030)
Naidong Wang (4725801)
Yalan Li (1696234)
Yingfan Zhang (508974)
Chengyu Liu (424209)
Yi Yang (116183)
Robert S. Adelstein (59194)
Aibing Wang (4318408)

February 2018

<p>(a). Schematic demonstrating the strategy for targeted integration of exogenous gene(s), wild typ...

Targeted disruption of <i>Mbd5</i> in mice.

Yarui Du (127339)
Bo Liu (127343)
Fan Guo (65416)
Guifang Xu (127349)
Yuqiang Ding (127352)
Yong Liu (6908)
Xin Sun (127355)
Guoliang Xu (67200)

October 2012

<p>(<b>A</b>) The strategy for the generation of a targeted <i>Mbd5</i> allele. Numbered black boxes...

Table_2_Precise and Rapid Validation of Candidate Gene by Allele Specific Knockout With CRISPR/Cas9 in Wild Mice.XLSX

Tianzhu Chao (6375851)
Zhuangzhuang Liu (6375854)
Yu Zhang (12946)
Lichen Zhang (5431988)
Rong Huang (298496)
Le He (577443)
Yanrong Gu (6375857)
Zhijun Chen (254277)
Qianqian Zheng (4811130)
Lijin Shi (6375860)
Wenping Zheng (6375863)
Xinhui Qi (6375866)
Eryan Kong (6375869)
Zhongjian Zhang (300392)
Toby Lawrence (151657)
Yinming Liang (270857)
Liaoxun Lu (179293)

February 2019

It is a tempting goal to identify causative genes underlying phenotypic differences among inbred str...

Inactivation of the tyrosinase locus in C57BL/6NTac ES cells and mice.

Branko Zevnik (257460)
Nathalie C. Uyttersprot (530957)
Ana V. Perez (530958)
Gerald W. M. Bothe (530959)
Heidrun Kern (27114)
Gunther Kauselmann (257458)

March 2014

<p>(A) Gene targeting scheme for the tyrosinase locus. A point mutation was introduced in exon 1 (C1...