Generation of conditional TAK1-deficient mice.

<p>(A) Schematic representation of the wild type, targeted, floxed and deleted <i>Map3k7</i> genomic locus, indicating BamHI, SacI and XbaI restriction sites used for Southern blot. The <i>Map3k7</i> locus comprises 17 exons; in the scheme the first 6 exons are represented. The probes used to verify homologous recombination at the 5′ and 3′ end are shown. Black arrows indicate loxP sites; red arrows indicate FRT sites. B, BamHI; S, SacI; X, XbaI. (B) Southern blot of genomic DNA isolated from BMDMs after digestion with XbaI and using the 3′ probe. (C) PCR analysis of genomic DNA isolated from FACS-sorted CD11b⁺F4/80⁺ resident peritoneal macrophages and CD11b⁺Gr1⁺ splenic neutrophils. (D) Colony formation by BM cells from <i>Map3k7</i>^Δmyel and <i>Map3k7</i>^flox/flox mice in response to M-CSF, G-CSF and GM-CSF. Data are shown as mean ± SEM of 4 mice per group and are representative of two independent experiments.