CD8+ and CD4+ T-lymphocyte Responses by Intracellular Cytokine Staining and Polychromatic Flow Cytometry.

<p>ICS was performed on a subset of samples from Groups 1–3, at baseline, visits 07/08 (2–4 weeks post HIVMAG (x3) prime) and visit 13 (2 weeks post Ad35-GRIN/Env boost). HIV-1-specific CD4+ (top panel) and CD8+ (bottom panel) T cell responses were evaluated by a 7-color ICS to assess the expression of IL-2, IFN-γ and TNF-α with peptides matched to the HIVMAG. Symbols show responses to MAG peptide pools; Env pool 1 = *, Env pool 2 = ○, Gag = </p><p></p><p></p><p><mn>●</mn></p><p></p><p></p>, Nef Tat Vif = ●, Pol pool 1 = Δ, Pol pool 2 = ▲. The y-axis shows the % expressing T cells on a half-log scale.<p>