HIV-1 antigens targeted by CD4+/CD8+ T-cell responses in NHP (2 weeks post last immunization).

<p>Magnitudes of p24-, p17-, RT- and Nef-specific CD40L⁺ CD4⁺ T-cell responses (panel A) and CD8⁺ T-cell responses (panel B) expressing IL-2, IFN- and/or TNF- were assessed at 2 weeks post last immunization using ICS. PBMCs were stimulated <i>in vitro</i> overnight with a pool of peptides covering the p24, p17, RT or Nef sequences. Individual data and box plots, showing the medians, upper and lower interquartiles and minimum and maximum values, are represented for each group. Pie charts show the mean proportions of CD40L⁺ CD4⁺ or CD8⁺ T cells specific to Nef, p17, p24 and RT (represented in light grey, medium grey, dark grey and black, respectively). The number in the center of each pie represents the geometric mean of the total percentage of cytokine-expressing CD40L⁺ CD4⁺ or CD8⁺ T cells at 2 weeks post last immunization.