IFN-γ and IL-2 production in response to HIV-1 Env antigen.

<p>Intracellular cytokine staining with flow cytometry analysis of IFN-γ - and IL-2 expressing, Env-specific CD8⁺/CD4⁺ splenic T cells stimulated with Env peptides. Mice were immunized with multi-clade Env constructs and protein boost groups, and splenocytes were collected and cultured. (A) Representative flow cytometry data for splenocytes harvested from mice immunized with multi-clade DNA followed protein boost and stimulated for five hours with the envelope peptide. (B&C) Bar graph showing the number of Env-specific IFN-γ and IL-2 expressing (B) CD4⁺ and (C) CD8⁺T-cell responses generated by <i>in vitro</i> stimulation as described in Materials & Methods. Results are the mean ± SD for 4 mice per group (<i>n = 4</i>). Data presented in this figure are from one experiment representative of two performed.