Intracellular cytokine staining of mouse splenocytes stimulated with HIV-1 Consensus B Env and Gag peptide pools.

<p>(A) Representative dot plot of CD4⁺ T cells expressing IL-2 and IL-4 after stimulation with HIV-1 Consensus B Env and Gag peptide pools. (B) Representative dot plot of CD4⁺ T cells expressing TNF-α and IFN-γ after stimulation with HIV-1 Consensus B Env and Gag peptide pools. (C) Relative levels of IL-2, IL-4, TNF-α, and IFN-γ in CD4⁺ T cells after stimulation with 2 μg/ml Gag peptide pool standardized to PBS control. (D) Relative levels of IL-2, IL-4, TNF-α, and IFN-γ in CD4⁺ T cells after stimulation with 2 μg/ml Env peptide pool standardized to PBS control. (E) Representative dot plot of CD8⁺ T cells expressing IL-2 and IL-4 after stimulation with HIV-1 Consensus B Env and Gag peptide pools. (F) Representative dot plot of CD8⁺ T cells expressing TNF-α and IFN-γ in CD8⁺ T cells after stimulation with HIV-1 Consensus B Env and Gag peptide pools. (G) Relative levels of IL-2, IL-4, TNF-α, and IFN-γ after stimulation with 2 μg/ml Gag peptide pool standardized to PBS control. (H) Relative levels of IL-2, IL-4, TNF-α, and IFN-γ in CD8⁺ T cells after stimulation with 2 μg/ml Env peptide pool standardized to Control. Error bars represent mean ± SEM (n = 6); * <i>p</i><0.05 (2-Way ANOVA and Bonferroni Post-hoc tests versus control group). # <i>p</i><0.05 (2-Way ANOVA and Bonferroni Post-hoc tests versus VLP Only and CALV(0)+VLP groups).