Figure 1

<p><i>Effect of γc cytokines on HIV specific T cell responses in a chronic progressor.</i> Fresh <i>ex vivo</i> PBMC from an HIV infected individual with chronic progression (Pt# 11) were incubated overnight in the following culture conditions: medium, IL-2 (20U/ml), IL-15 (20 ng/ml), IL-15+IL-7 (20 ng/ml each), or IL-4 (20 ng/ml). The following day, cells were washed twice, resuspended in plain medium and then stimulated with control antigen (DMSO) or a <i>gag</i> peptide pool in the presence of Monensin, and then stained for intracellular cytokines. Shown are dot plots of <i>gag</i> or control antigen stimulated samples from PBMC in respective culture conditions, for CD4⁺ T cells (a) or CD8⁺ T cells (b). The upper right numbers in the right hand panels indicate the % of HIV <i>gag</i> specific IFN-γ producing CD4⁺ or CD8⁺ T cells after subtraction from DMSO controls (left panel).