Differentiation assay of the mES-<i>Tbx1</i>Tet^OFF cell line.

<p>A: Scheme of experimental protocol. B: qRT-PCR assay of <i>Tbx1</i> expression in uninduced (+Tet) mES-<i>Tbx1</i>Tet ^OFF cells. A peak of expression is evident at day 6. C: Schematic representation of the experimental strategy used for pulse <i>Tbx1</i> expression. D: qRT-PCR analysis of <i>Tbx1</i> expression at day 6 after 24hrs without tetracycline. E: Immunofluorescence with an anti-P-H3 antibody on two colonies of mES-<i>Tbx1</i>Tet ^OFF cells with and without tetracycline at day 6. F: graphic representation of quantitative evaluation of mitotic activity using flow cytometry.