<i>Tbx1</i> occupies the TBEs of <i>Wnt5a</i> in P19Cl6 cells.

<p>Chromatin immunoprecipitation (ChIP) assays of P19Cl6 using an anti-Tbx1 antibody. (A) Standard PCR results using primers for the TBE1–2 and the TBE3 regions of <i>Wnt5a</i>. Amplification is clearly visible from the Tbx1-immunoprecipitated material and from the input sample but not from the IgG-immunoprecipitated material (negative control). (B) Quantitative ChIP assay (q-ChIP) on a similar experiment as the one shown in (A) but followed by quantitative real-time PCR. (C) Q-ChIP assay of E9.5 mouse embryos using an anti-Tbx1 antibody. The enrichment of TBE1–2 and TBE3 regions is evaluated compared to an internal control, which is a primer pair amplifying an ORF-free segment of mouse chromosome 14, and expressed as percentage of input. Values are from 3 experiments (mean±S.D.).