<p>A: schematic representation of experimental strategy. B: Examples of RT-PCR analysis on VEGFR2+ cells in absence and in presence of <i>Tbx1</i> pulse. C: Color map panels of marker positivity of individual clones with or without <i>Tbx1</i> pulse. D: Graphic representation of clone multiplicity, based on the number of positive markers with and without tetracycline. Blue represents the percentage of clones that were positive for all three markers; Red represents the percentage of clones positive for two markers; Green represents the percentage of clones that are positive for one marker.</p
<p>(A) iPSC clones generated from BJ fibroblasts and characterized for the presence of pluripotent m...
<p>(A) Schematic diagram of the plasmids and methodology used to create blasticidin-resistant (Bsd<s...
Clonal analysis is important for many areas of hematopoietic stem cell research, including in vitro ...
<p>A: Scheme of experimental protocol. B: qRT-PCR assay of <i>Tbx1</i> expression in uninduced (+Tet...
<p>(A) Anti-viral clones (identified by tetramer sorting followed by NGS). Each dot represents a dif...
<p>Gray and black bars denote the expression of the indicated genes for non-treated and treated with...
<p>A: qRT-PCR assay of <i>Vegfr2</i> expression of cells bound to magnetic beads (VEGFR2+ cells) and...
A: Dot plot showing the percentage of bone marrow plasma cells in samples by success or failure of c...
<p>For FC analysis, cells from replicated samples were collected, fixed and stained at 6, 24, 48 and...
<p>Conceptually, a clone is a group of genetically identical cells derived from a single common ance...
<p>A. Overview of the method. In short, a defined number of cells was harvested and lysed (steps 1 a...
<p>(A) Representation of the infectious clones. (B–C) Vero cells (I–IV) and SH-SY5Y cells (V–VIII) w...
<p><i>In vitro</i> generated T-cells were analyzed by spectratyping, i.e. quantitative RT-PCR expres...
<p>(<b>A</b>) Constructs utilized to generate infectious particles for the transduction of SupT1 cel...
<p>A) Basal-like xenograft cells. B) Luminal-like xenograft cells. A and B displays pseudo-color dot...
<p>(A) iPSC clones generated from BJ fibroblasts and characterized for the presence of pluripotent m...
<p>(A) Schematic diagram of the plasmids and methodology used to create blasticidin-resistant (Bsd<s...
Clonal analysis is important for many areas of hematopoietic stem cell research, including in vitro ...
<p>A: Scheme of experimental protocol. B: qRT-PCR assay of <i>Tbx1</i> expression in uninduced (+Tet...
<p>(A) Anti-viral clones (identified by tetramer sorting followed by NGS). Each dot represents a dif...
<p>Gray and black bars denote the expression of the indicated genes for non-treated and treated with...
<p>A: qRT-PCR assay of <i>Vegfr2</i> expression of cells bound to magnetic beads (VEGFR2+ cells) and...
A: Dot plot showing the percentage of bone marrow plasma cells in samples by success or failure of c...
<p>For FC analysis, cells from replicated samples were collected, fixed and stained at 6, 24, 48 and...
<p>Conceptually, a clone is a group of genetically identical cells derived from a single common ance...
<p>A. Overview of the method. In short, a defined number of cells was harvested and lysed (steps 1 a...
<p>(A) Representation of the infectious clones. (B–C) Vero cells (I–IV) and SH-SY5Y cells (V–VIII) w...
<p><i>In vitro</i> generated T-cells were analyzed by spectratyping, i.e. quantitative RT-PCR expres...
<p>(<b>A</b>) Constructs utilized to generate infectious particles for the transduction of SupT1 cel...
<p>A) Basal-like xenograft cells. B) Luminal-like xenograft cells. A and B displays pseudo-color dot...
<p>(A) iPSC clones generated from BJ fibroblasts and characterized for the presence of pluripotent m...
<p>(A) Schematic diagram of the plasmids and methodology used to create blasticidin-resistant (Bsd<s...
Clonal analysis is important for many areas of hematopoietic stem cell research, including in vitro ...