Resistance of swine and human origin IAV strains conferred to poly I:C induced innate response.

<p>(A-C) Analysis of IFN-λ3 (A), IFN-λ1 (B), and IFN-β (C) gene expressions in response to poly I:C treatment by primary pAECs is presented. Cells were treated with 50 μg/ml poly I:C and the total cellular RNA was extracted at indicated time post treatment. Interferon gene expression was analyzed by RT-qPCR. Data were normalized to β-actin gene expression and represented as fold change over saline (vehicle) treated cells. The experiment was performed in duplicate using cells derived from two donor animals. (D) Titers of IAV pH1N1 CA/09 and H1N1 IL/08 in pAECs pretreated with poly I:C or saline (vehicle) for 24 h prior to infection (0.01 MOI). Virus titers in culture supernatants were assessed 24 h p.i. by TCID₅₀ assay. Data are average of two independent experiments performed in duplicate. ND; not detected. *p <0.05.