HRV1B induced interferon response in PBECs and its comparison with other stimuli.

<p>PBECs were infected with HRV1B for 4(n = 4). The mRNA expression of ISGs was determined with qPCR and fold changes were calculated with the 2^−ΔΔCt method (A). Cells were infected with RSV (MOI-1, 1 h) or HRV1B (4 h). After infection, virus-containing medium was removed and replaced with fresh medium. Additionally, cells were stimulated with poly(I:C)/LyoVec (500 ng/ml) for 24 h (n = 3). Then cells were collected and IFNβ/λ1 mRNA expression was determined by qPCR and relative amount of mRNA was calculated with the 2^−ΔCt method (B). *, p<0.05 control vs rest of the conditions.