Conditioned medium from Poly I:C stimulated cultures increases ISG and IFN-λ3 expression in pAECs.

<p>Conditioned medium (CM) was prepared by treating pAECs for 24 h with 50 μg/ml poly I:C (poly I:C CM) or with saline (mock CM) and diluted in pAEC growth medium at indicated concentrations. Control medium was prepared by adding poly I:C (50 μg/ml) in pAEC growth medium (poly I:C M) and similarly diluted to compare the direct effect of residual poly I:C in the conditioned medium. pAECs were treated at indicated concentration of conditioned medium for 12 h. (A) Analysis of ISG expression in pAECs treated with conditioned medium from poly I:C treated cells. Total RNA was analyzed for the expression of Mx1 and OAS1 by RT-qPCR. Fold change in gene expression over growth medium and normalized to β-actin is presented. (B) Analysis of IFN expression in pAEC treated with conditioned medium (Poly I:C CM), Mock CM, or control medium (Poly I:C M), as described above, demonstrating fold change in expression of IFN-λ3, IFN-λ1 and IFN-β genes over growth medium and normalized to β-actin. *p <0.05.