Role of miR-155 in the KLF2-induced inhibition of macrophage activation.

<p>To further confirm the role of miR155 in the KLF2-induced inhibition of macrophage inflammatory responses, WT macrophages and macrophages infected with EV or with viruses containing Ad-KLF2, siRNA-KLF2, or nonspecific control siRNA (NS) were cultured for 24 h. Using Lipofectamine 2000, mimic-miR-155 or anti-miR-155 was transfected into macrophages. Unless otherwise stated, cells were incubated for 24 hours post-transfection and then exposed to ox-LDL (50 μg/ml) for an additional 24 hours. Culture supernatants were evaluated by ELISA to determine MCP-1 and IL-6 levels. Data are presented as means ± S.D. for three independent experiments. The effects of miR-155 over-expression on MCP-1 and IL-6 expression in ox-LDL-stimulated macrophages were examined (A). The impact of miR-155 over-expression on IL-10 expression in ox-LDL-stimulated macrophages was evaluated (B). The effects of silencing miR-155 on MCP-1 and IL-6 expression in ox-LDL stimulated macrophages were assessed (C). The impact of silencing miR-155 on IL-10 expression in ox-LDL stimulated macrophages was examined (D) (# and δ indicate p<0.05 and p>0.05, respectively, for the indicated comparisons).