Inhibition of miR-155 in TLR3-stimulated macrophages increases their susceptibility to HIV-1 infection.

<p>MDMs from seven different donors were transfected for 24 h (LipoRNAiMax, Invitrogen) with 30 nM anti-miR-155 antagomir or a scrambled control that doesn't target any known human miRNA (anti-miR-scr) (Applied Biosystems), and then stimulated with poly(I∶C) (A and B) or Imiquimod (C and D) for 16 h, before either infecting with BaL pseudotypes (A, C) or isolating RNA for miR-155 quantitation (B, D). Infection was evaluated in 8 replicates as percent luc activity compared to untreated, unstimulated MDMs (mean ± SD). Relative miR-155 levels were evaluated as in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002937#ppat-1002937-g003" target="_blank">Figure 3</a> and results are shown as fold-change (mean ± SD) with respect to the untreated, unstimulated MDMs.