miR-155 promotes pro-inflammatory cytokine production during <i>F.n.</i> infection.

<p>(A) PBM were transfected with vector control or miR-155 overexpression construct. 22 hours post-transfection, RNA was extracted and miR-155 over-expression was verified by qRT-PCR. (B) SHIP expression was assayed from the samples in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0008508#pone-0008508-g005" target="_blank">Figure 5A</a>. (C–D) 14 hours post-transfection PBM were infected with <i>F.n.</i> at an MOI of 50 for 8 hours and (C) TNFα and (D) IL-6 measured in supernatants. (E) Wild-type and miR-155^−/− macrophages were assayed for miR-155 expression. (F) SHIP expression was assayed by qRT-PCR from the samples in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0008508#pone-0008508-g005" target="_blank">Figure 5E</a>. (G) Wild-type and miR-155^−/− macrophages were infected with <i>F.n.</i> at an MOI of 50 for eight hours. IL-6 in the supernatant was measured by ELISA. All experiments were performed in triplicate. Graphs represent the mean and standard deviation of samples from three independent experiments. Data were analyzed by a paired Student <i>t</i> test. An asterisk (*) indicates a <i>p</i>-value<0.05.