Species-specificity of RF4 as determined by PCR.

<p>Agarose gels showing specific amplification of <i>L</i>. <i>loa</i> RF4 <b>(A)</b> or a conserved 244 bp actin gene fragment <b>(B)</b> from WGA <i>L</i>. <i>loa</i> (WGA Ll), genomic <i>L</i>. <i>loa</i> (g Ll), <i>W</i>. <i>bancrofti</i> (Wb), <i>O</i>. <i>volvulus</i> (Ov), <i>B</i>. <i>malayi</i> (Bm), <i>A</i>. <i>albopictus</i> (Aa), and <i>H</i>. <i>sapiens</i> (Hs) DNA. PCR <b>(A)</b> and low molecular weight <b>(B)</b> markers (MWM) were used (New England Biolabs).