Internal transcribed spacer 1 (<i>ITS1</i>) gene amplicons from <i>Leishmania</i> DNA and restriction fragment length polymorphism (RFLP) analyses.

<p>Silver-stained 5% polyacrylamide gels showing: <b>A</b>) ITS1-PCR: Lane M—Promega standard molecular weight ladder (100 bp), Lane 1—positive control—amplicon from DNA of <i>Leishmania infantum</i> promastigotes, Lanes 2–15—amplicons from DNA sandfly samples collected in Caxias, Maranhão, Brazil, Lane 16—negative control; <b>B</b>) ITS1-PCR-RFLP (<i>Hae</i>III digestion): Lane M—Invitrogen standard molecular weight ladder (25 bp), Lanes I-IX—DNA from positive controls, I—<i>L</i>. <i>amazonensis</i>, II—<i>L</i>. <i>mexicana</i>, III–<i>L</i>. <i>guyanensis</i>, IV- <i>L</i>. <i>braziliensis</i>, V–<i>L</i>. <i>lansoni</i>, VI–<i>L</i>. <i>naiffi</i>, VII—<i>L</i>. <i>shawi</i>, VIII—<i>L</i>. <i>infantum</i>, IX—<i>L</i>. <i>infantum/L</i>. <i>braziliensis</i>, Lanes 3–55—DNA from phlebotomine samples <b>C</b>) ITS1-PCR-RFLP (<i>Hae</i> III digestion): Lane M: Invitrogen standard molecular weight ladder (25 bp), Lanes 56–209—DNA from phlebotomine samples.