<p>RFLP products were resolved in 2% agarose gels, stained with ethidium bromide; A. <i>TLR4</i> 896 A>G SNP; B. <i>TLR4</i> 1196 C>T SNP; C. <i>TLR9</i> 2848 G>A SNP. The numbers on the side of electropherograms indicate the lengths of separated DNA fragments. M—50 bp DNA marker; Ud—undigested PCR product; AA, AG, GG, GA, CC, CT or TT—genotypes at analyzed <i>TLR</i> SNPs.</p
<p>(A) <i>IL</i>-8 +781C/T SNP: lane M, 100 bp marker ladder, lanes 1–4, samples, the 203 bp bands a...
<p><i>P</i> value was calculated by a χ<sup>2</sup>–test 3×2 contingency table (df = 2).</p
<p><sup>a</sup> No., number.</p><p><sup>b</sup> SNP, single nucleotide polymorphism.</p><p><sup>c</s...
<p>PCR-based RFLP’s were performed with genomic DNA from each participant. Representative agarose ge...
<p>Reverse strand sequences were determined for all the analyzed amplicons of <i>TLR</i> gene fragme...
<p>Representative agarose gels showing PCR-RFLP analysis of different single nucleotide polymorphism...
<p>Gel image: 1 and 2, heterozygous TC genotype (192, 327, 490 bp); 3 and 4, TT genotype (192, 327 b...
<p>(1a) 346 bp DNA fragment was amplified by PCR, incubated with Dde1 restriction endonuclease for 1...
<p>Gel image: A) rs3775290: 1. wild type CC, 2. heterozygous CT, 3. homozygous recessive TT; rs37752...
<p>(A) Representative ethidium bromide stained (15%) non-denaturing polyacrylamide gel (PAGE) showin...
Background: Restriction fragment length polymorphism (RFLP) is a common molecular assay used for gen...
<p>A: Locations of SNP rs5896 and restriction sites of <i>Nco</i>I on amplified DNA fragment (733 bp...
<p>PCR product was digested by restriction enzyme and visualized on a 2.5% agarose gel. a −589C/T po...
<p>F refers to the forward primer, and R refers to the reverse primer for a particular locus.</p>a<p...
<p>(A) RFLP patterns of SNP Thr307Ala visualized on 2.8% agarose gel. (B) Confirmation of RFLP by DN...
<p>(A) <i>IL</i>-8 +781C/T SNP: lane M, 100 bp marker ladder, lanes 1–4, samples, the 203 bp bands a...
<p><i>P</i> value was calculated by a χ<sup>2</sup>–test 3×2 contingency table (df = 2).</p
<p><sup>a</sup> No., number.</p><p><sup>b</sup> SNP, single nucleotide polymorphism.</p><p><sup>c</s...
<p>PCR-based RFLP’s were performed with genomic DNA from each participant. Representative agarose ge...
<p>Reverse strand sequences were determined for all the analyzed amplicons of <i>TLR</i> gene fragme...
<p>Representative agarose gels showing PCR-RFLP analysis of different single nucleotide polymorphism...
<p>Gel image: 1 and 2, heterozygous TC genotype (192, 327, 490 bp); 3 and 4, TT genotype (192, 327 b...
<p>(1a) 346 bp DNA fragment was amplified by PCR, incubated with Dde1 restriction endonuclease for 1...
<p>Gel image: A) rs3775290: 1. wild type CC, 2. heterozygous CT, 3. homozygous recessive TT; rs37752...
<p>(A) Representative ethidium bromide stained (15%) non-denaturing polyacrylamide gel (PAGE) showin...
Background: Restriction fragment length polymorphism (RFLP) is a common molecular assay used for gen...
<p>A: Locations of SNP rs5896 and restriction sites of <i>Nco</i>I on amplified DNA fragment (733 bp...
<p>PCR product was digested by restriction enzyme and visualized on a 2.5% agarose gel. a −589C/T po...
<p>F refers to the forward primer, and R refers to the reverse primer for a particular locus.</p>a<p...
<p>(A) RFLP patterns of SNP Thr307Ala visualized on 2.8% agarose gel. (B) Confirmation of RFLP by DN...
<p>(A) <i>IL</i>-8 +781C/T SNP: lane M, 100 bp marker ladder, lanes 1–4, samples, the 203 bp bands a...
<p><i>P</i> value was calculated by a χ<sup>2</sup>–test 3×2 contingency table (df = 2).</p
<p><sup>a</sup> No., number.</p><p><sup>b</sup> SNP, single nucleotide polymorphism.</p><p><sup>c</s...