<p>Cells overexpressing LPA<sub>1–3</sub> receptors were preincubated for 15 min in the presence of the PKC inhibitor, bisindolylmaleimide I (BIM), and then subjected to the desensitization protocols (indicated under the Experimental section and in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0140583#pone.0140583.g002" target="_blank">Fig 2</a>), using 1 μM PMA or 1 μM LPA. Cells were challenged with 1 μM LPA and the increase in intracellular free calcium concentration was determined. Plotted are the increases in calcium as the percentage of that obtained in cells preincubated without any agent as mean ± S. E. M. of 6–8 experiments using different cell preparations. *p < 0.001 vs. baseline.</p
Addition of monoclonal antibodies (UCHT1) directed against the antigen receptor/CD3 complex or the a...
Addition of monoclonal antibodies (UCHT1) directed against the antigen receptor/CD3 complex or the a...
Treatment of HL-60 cells with thapsigargin, a microsomal Ca2+/ATPase inhibitor, led to depletion of ...
<p>Cells overexpressing LPA<sub>1–3</sub> receptors were preincubated overnight with 1 μM PMA, and t...
<p>Cells overexpressing LPA<sub>1</sub> (black, circles), LPA<sub>2</sub> (blue, squares) or LPA<sub...
<p>Cells overexpressing LPA<sub>1</sub> (panel A, black), LPA<sub>2</sub> (panel B, blue) or LPA<sub...
<p>Cells overexpressing LPA<sub>1</sub> (panel A, black symbols and lines), LPA<sub>2</sub> (panel B...
Treatment of HL-60 cells with thapsigargin, a microsomal Ca2+/ATPase inhibitor, led to depletion of ...
<p>Cells overexpressing LPA<sub>1</sub> (panel A, black bars), LPA<sub>2</sub> (panel B, blue bars) ...
<p>Wild type C9 cells (panel A) or overexpressing LPA<sub>1</sub> (panel B), LPA<sub>2</sub> (panel ...
1 The effect of the specific protein kinase C (PKC) inhibitor, GF109203X, were measured on the cytop...
<p>Calcium transients from hMC (A) and LAD2 cells (B) with cys-LTs (500 nM) in presence or absence o...
1 The effect of the specific protein kinase C (PKC) inhibitor, GF109203X, were measured on the cytop...
<p>(A) Calcium flux in response to the indicated concentrations of LPA in control (grey tracing) or ...
Addition of monoclonal antibodies (UCHT1) directed against the antigen receptor/CD3 complex or the a...
Addition of monoclonal antibodies (UCHT1) directed against the antigen receptor/CD3 complex or the a...
Addition of monoclonal antibodies (UCHT1) directed against the antigen receptor/CD3 complex or the a...
Treatment of HL-60 cells with thapsigargin, a microsomal Ca2+/ATPase inhibitor, led to depletion of ...
<p>Cells overexpressing LPA<sub>1–3</sub> receptors were preincubated overnight with 1 μM PMA, and t...
<p>Cells overexpressing LPA<sub>1</sub> (black, circles), LPA<sub>2</sub> (blue, squares) or LPA<sub...
<p>Cells overexpressing LPA<sub>1</sub> (panel A, black), LPA<sub>2</sub> (panel B, blue) or LPA<sub...
<p>Cells overexpressing LPA<sub>1</sub> (panel A, black symbols and lines), LPA<sub>2</sub> (panel B...
Treatment of HL-60 cells with thapsigargin, a microsomal Ca2+/ATPase inhibitor, led to depletion of ...
<p>Cells overexpressing LPA<sub>1</sub> (panel A, black bars), LPA<sub>2</sub> (panel B, blue bars) ...
<p>Wild type C9 cells (panel A) or overexpressing LPA<sub>1</sub> (panel B), LPA<sub>2</sub> (panel ...
1 The effect of the specific protein kinase C (PKC) inhibitor, GF109203X, were measured on the cytop...
<p>Calcium transients from hMC (A) and LAD2 cells (B) with cys-LTs (500 nM) in presence or absence o...
1 The effect of the specific protein kinase C (PKC) inhibitor, GF109203X, were measured on the cytop...
<p>(A) Calcium flux in response to the indicated concentrations of LPA in control (grey tracing) or ...
Addition of monoclonal antibodies (UCHT1) directed against the antigen receptor/CD3 complex or the a...
Addition of monoclonal antibodies (UCHT1) directed against the antigen receptor/CD3 complex or the a...
Addition of monoclonal antibodies (UCHT1) directed against the antigen receptor/CD3 complex or the a...
Treatment of HL-60 cells with thapsigargin, a microsomal Ca2+/ATPase inhibitor, led to depletion of ...