Production of surface MshA pili is MshE and PilT dependent and repressed by CdgJ.

<p>A. Surface MshA pilin production determined via ELISA with MshA-specific antibody. Two biological replicates were assayed in quadruplicate and normalized to the average of the WT strain. Oneway ANOVA followed by Bonferroni’s Multiple Comparison test. (ns = not significant, * p≤0.05, ** p≤0.01) B. Western blots detect MshA production from cell pellets using α-MshA antibody. Blots were performed in triplicate, with one representative image included. C. Densitometry of Western blots quantified MshA production in various strains. Densitometry analysis of blots was performed in triplicate and detection was normalized to the WT sample on each blot. Statistical significance determined using a one sample t-test to compare to WT. (*** p≤0.001).