<i>mshA</i>, <i>mshE</i>, and <i>pilT</i> mutants are impaired in biofilm formation.

<p>A. Three-dimensional biofilm structures of WT, Δ<i>mshA</i>, Δ<i>mshE</i>, Δ<i>pilT</i>, and Δ<i>pilU</i> strains formed 24 h post inoculation in a flow cell system. Scale bar represents 20μm. B. COMSTAT quantitative analysis of biomass, average thickness, and surface coverage of biofilms from A. Three images from each of two independent experiments were analyzed. Significance was determined with a Oneway ANOVA followed by Dunnett’s Multiple Comparison test comparing to WT. (ns = not significant, *** p≤0.001) C. Three-dimensional biofilm structures of WT, Δ<i>cdgJ</i>, Δ<i>cdgJmshA</i>, Δ<i>cdgJmshE</i>, and Δ<i>cdgJpilT</i>, strains formed after 24 h post inoculation in a flow cell system. Scale bar represents 20μm. D. COMSTAT quantitative analysis of biomass, average thickness, and surface coverage of biofilms from C. Three images from each of two independent experiments were analyzed. Significance was determined with a Oneway ANOVA followed by Dunnett’s Multiple Comparison test comparing to Δ<i>cdgJ</i>. (ns = not significant, * p≤0.05, *** p≤0.001).