<p>(<b>A</b>) Microtiter-plate biofilm assay of EGD WT and the isogenic deletion mutants for <i>prfA</i>, <i>inlA</i>, <i>inlB</i>, <i>hly</i> and <i>actA</i> genes. Biofilm biomass formed in 24 h at the surface of the wells and stained by crystal violet (bottom view of the wells) was quantified by densitometry. (<b>B</b>) Comparison of WT <i>versus</i> Δ<i>actA</i> mature biofilm formed in continuous-flow microfermentors in 42 h. Biofilm biomasses formed on spatula were quantified by spectrophotometry and by CFUs enumeration. (<b>C</b>) Comparison of biofilms formed in microtiter-plate in 24 h after expression of <i>actA</i> in <i>Li</i>. (<b>D</b>) Observation of EGD WT <i>versus</i> Δ<i>actA</i> biofilms formed on glass slides by confoca...
<p>A. Three-dimensional biofilm structures of WT, Δ<i>mshA</i>, Δ<i>mshE</i>, Δ<i>pilT</i>, and Δ<i>...
Most microorganisms in nature subsist as heterogeneous surface-associated communities called biofilm...
<p>Biofilm production by the wild type strain 407 (wt) and by the 407 mutants <i>spo0A</i>, <i>abrB<...
<p>Kinetics of biofilm development for different strains was analyzed by the microtiter assay. Optic...
<p>Microtiter plates were washed and stained with crystal violet and biofilms visualized in (A) and ...
<p>Kinetics of biofilm development for the different strains was analyzed by the microtiter assay. O...
<p><b>(A)</b> Single row of a microtiter plate showing the relative intensity of crystal violet-stai...
<p>Biofilm quantification using crystal violet assay showed the variability in biofilm formation bet...
<p>(A) Microtiter plate assay: Biofilm was measured each day for cells inoculated in TSB broth with ...
<p>Data shown are means and SD of absorbance values, compared by a two tail <i>t</i> test. NTHi5a an...
<p>The formation of <i>rdar</i> morphotype in wild type, single and double <i>dsb</i> mutants grown ...
<p><b>(A)</b> in plastic 96-well plates measured by the crystal violet assay; <b>(B)</b> Spectrophot...
<p>a) Visualisation of three day old biofilms attached to the bottom of the individual wells in a 24...
Biofilm formation is the irreversible attachment and growth of a population of microorganisms on a s...
<p>Biofilm formation was quantified by measuring the absorbance at 595 nm after crystal violet stain...
<p>A. Three-dimensional biofilm structures of WT, Δ<i>mshA</i>, Δ<i>mshE</i>, Δ<i>pilT</i>, and Δ<i>...
Most microorganisms in nature subsist as heterogeneous surface-associated communities called biofilm...
<p>Biofilm production by the wild type strain 407 (wt) and by the 407 mutants <i>spo0A</i>, <i>abrB<...
<p>Kinetics of biofilm development for different strains was analyzed by the microtiter assay. Optic...
<p>Microtiter plates were washed and stained with crystal violet and biofilms visualized in (A) and ...
<p>Kinetics of biofilm development for the different strains was analyzed by the microtiter assay. O...
<p><b>(A)</b> Single row of a microtiter plate showing the relative intensity of crystal violet-stai...
<p>Biofilm quantification using crystal violet assay showed the variability in biofilm formation bet...
<p>(A) Microtiter plate assay: Biofilm was measured each day for cells inoculated in TSB broth with ...
<p>Data shown are means and SD of absorbance values, compared by a two tail <i>t</i> test. NTHi5a an...
<p>The formation of <i>rdar</i> morphotype in wild type, single and double <i>dsb</i> mutants grown ...
<p><b>(A)</b> in plastic 96-well plates measured by the crystal violet assay; <b>(B)</b> Spectrophot...
<p>a) Visualisation of three day old biofilms attached to the bottom of the individual wells in a 24...
Biofilm formation is the irreversible attachment and growth of a population of microorganisms on a s...
<p>Biofilm formation was quantified by measuring the absorbance at 595 nm after crystal violet stain...
<p>A. Three-dimensional biofilm structures of WT, Δ<i>mshA</i>, Δ<i>mshE</i>, Δ<i>pilT</i>, and Δ<i>...
Most microorganisms in nature subsist as heterogeneous surface-associated communities called biofilm...
<p>Biofilm production by the wild type strain 407 (wt) and by the 407 mutants <i>spo0A</i>, <i>abrB<...