Mutations in <i>mshA</i>, <i>mshE</i>, and <i>pilT</i> increase motility.

<p>A. The diameters of migration zones of the WT and mutants were measured after 16 h of incubation at 30°C on LB soft agar motility plates and normalized to the motility of the WT strain. Four biological replicates were performed in quadruplicate. Statistical significance determined with Oneway ANOVA followed by Dunnett’s multiple comparison test comparing to the WT strain. (ns—not significant, *** p≤0.001) B. Expression of <i>mshA</i> reduces motility in soft agar. The diameters of migration zones were measured after 16 hours of growth at 30°C with or without IPTG induction and normalized to the motility of the WT strain. Two biological replicates were performed in triplicate. Induced strains compared to uninduced strains with Student’s t-test. (ns—not significant, *** p≤0.001).