Swimming and swarming motility phenotypes of single gene deletion mutants putatively decreasing motility.

<p>Motility phenotypes of mutants were analyzed on soft-agar plates (0.3% agar) after 4 hours incubation at 37°C for swimming motility. Swarming motility was analyzed on plates containing 0.6% agar after 9 hours incubation at 37°C. (A) Representative motility plates for each mutant tested (TH6622, EM824, EM1480, EM1508, EM1511, EM1512, EM1688, EM1690, EM2384, EM2385, EM2590, EM2591, and EM2605). The diameter of the motility swarm and the swarming area were measured and normalized to the wildtype. (B) Quantified relative motility. Biological replicates are shown as individual data points and analyzed by the Student’s <i>t</i> test. Asterisks indicate a significantly different motility phenotype (*** = P<0.001).