Fluorescence microscopy of the indicated strains. Phase images (top) show cell boundaries and fluorescence images (bottom) show AF488-mal labeled pili. Each sample is shown before (-) and after (+) induction. Scale bar = 4 μm. Data are representative of three independent experiments. (TIFF)</p
The opportunistic pathogen Pseudomonas aeruginosa expresses polar type IV pili (TFP), which are resp...
Bacterial type IV pili are critical for diverse biological processes including horizontal gene trans...
Type IV pili are dynamic cell surface appendages found throughout the bacteria. The ability of these...
(A) Quantification of piliation in strains imaged before and after induction with 0.2% arabinose + 1...
(A) Representative images of cells where mf-Lon was (“+”; 0.2% arabinose) or was not (“-”) induced. ...
(A) Representative images of piliated cells containing the indicated mutations to their retraction m...
(A) Western blot analysis of MshA protein levels in parent and ΔpilT strains. Samples represent eith...
(A) The speed of MSHA T4P extension was determined in strains containing native motor ATPases (paren...
In wildtype cells (left), PilT promotes initiation of pilus extension, potentially by maturing the m...
(A) Representative montage of piliated AF488-mal labeled cells for the pilus system indicated. Strai...
<p>A. Surface MshA pilin production determined via ELISA with MshA-specific antibody. Two biological...
(A) Predicted structure of MshA, generated using AlphaFold [64,65]. Residues mutated in the suppress...
(A) Quantification of piliation in strains with altered extension motor regulation. Strains that ret...
<p>Transmission Electron Microscopy of negative stained cells in exponential growth reveals MshA pil...
<p>A. Three-dimensional biofilm structures of WT, Δ<i>mshA</i>, Δ<i>mshE</i>, Δ<i>pilT</i>, and Δ<i>...
The opportunistic pathogen Pseudomonas aeruginosa expresses polar type IV pili (TFP), which are resp...
Bacterial type IV pili are critical for diverse biological processes including horizontal gene trans...
Type IV pili are dynamic cell surface appendages found throughout the bacteria. The ability of these...
(A) Quantification of piliation in strains imaged before and after induction with 0.2% arabinose + 1...
(A) Representative images of cells where mf-Lon was (“+”; 0.2% arabinose) or was not (“-”) induced. ...
(A) Representative images of piliated cells containing the indicated mutations to their retraction m...
(A) Western blot analysis of MshA protein levels in parent and ΔpilT strains. Samples represent eith...
(A) The speed of MSHA T4P extension was determined in strains containing native motor ATPases (paren...
In wildtype cells (left), PilT promotes initiation of pilus extension, potentially by maturing the m...
(A) Representative montage of piliated AF488-mal labeled cells for the pilus system indicated. Strai...
<p>A. Surface MshA pilin production determined via ELISA with MshA-specific antibody. Two biological...
(A) Predicted structure of MshA, generated using AlphaFold [64,65]. Residues mutated in the suppress...
(A) Quantification of piliation in strains with altered extension motor regulation. Strains that ret...
<p>Transmission Electron Microscopy of negative stained cells in exponential growth reveals MshA pil...
<p>A. Three-dimensional biofilm structures of WT, Δ<i>mshA</i>, Δ<i>mshE</i>, Δ<i>pilT</i>, and Δ<i>...
The opportunistic pathogen Pseudomonas aeruginosa expresses polar type IV pili (TFP), which are resp...
Bacterial type IV pili are critical for diverse biological processes including horizontal gene trans...
Type IV pili are dynamic cell surface appendages found throughout the bacteria. The ability of these...