Differential regulation of IL-23 and IL-12 productions in <i>T</i>. <i>gondii</i>-infected THP-1 cells upon pretreatment with PI3K inhibitors.

<p>PI3K inhibitor LY294002 (LY) or wortmannin (WM) was added to THP-1 cells at the concentrations indicated at 1 h before infection with <i>T</i>. <i>gondii</i> at a MOI of 1. The supernatants were harvested after 18 h for cytokine assessment using ELISA. One representative experiment performed in triplicate is shown. The IL-23 (A) or IL-12 (B) levels (mean ± SD) following stimulation with <i>T</i>. <i>gondii</i>, as well as IL-23 and IL-12 production in the presence of the inhibitor, are shown. The solvent control was 0.1% DMSO. * <i>P</i><0.05 compared with DMSO-treated <i>T</i>. <i>gondii</i>-infected group.