IFN-γ, IL-12 and TNF-α expression in <i>T. gondii</i>-infected B6 and PKR^−/− mice.

<p>B6 and PKR^−/− mice were infected i.p. with 10 cysts of the ME49 strain of <i>T. gondii</i>. Mice were euthanized at different times post-infection. <i>A</i>, Comparison of IFN-γ, IL-12 and TNF-α RNA levels between B6 and PKR^−/− mice. RNA was isolated from organs and levels of IFN-γ, IL-12 and TNF-α were examined by quantitative PCR and normalized against the levels of 18s rRNA. Increase in mRNA levels was calculated by comparing to uninfected organs. <i>B</i>, Concentrations of IFN-γ, IL-12 and TNF-α were measured by ELISA in sera collected at 7 days post-infection. <i>C</i>, Splenocytes were obtained at day 7 post-infection and incubated with or without TLA (10 µg/ml). IFN-γ IL-12 and TNF-α were measured by ELISA. Results are shown as the mean ± SEM and are representative of 3 independent experiments. *p<0.05.