PKR^−/− mice exhibit unimpaired composition of lymphoid organs, expansion of IFN-γ⁺ T cells and anti-<i>T. gondii</i> IgG production.

<p><i>A</i>, single cell suspensions of splenocytes from B6 and PKR^−/− mice were stained with anti-CD3, anti-CD4, anti-CD8, anti-CD11b, anti-CD45R, anti-CD49d (DX5) mAb followed by flow cytometric analysis. <i>B–F</i>, B6 and PKR^−/− mice were infected i.p. with 10 cysts of the ME49 strain of <i>T. gondii</i>. Splenocytes obtained at day 7 (<i>B, C</i>) and brain mononuclear cells obtained at 4 weeks post-infection (<i>D, E</i>) were cultured with anti-CD3 mAb and CD4⁺ and CD8⁺ cells were analyzed for intracellular IFN-γ by flow cytometry. <i>F</i>, Anti-<i>T. gondii</i> IgG detection in sera collected at 4 weeks post-infection. O.D. in sera from uninfected mice were ≤0.06. Results are shown as the mean ± SEM and are representative of 3 independent experiments.