Mushroom body abnormalities seen in <i>Nipped-B</i> heterozygotes.

<p>Immunofluorescence images of 2 to 4 day old adult brains are shown. Mushroom bodies (MBs) are labeled by anti-FasII (shown in red) and <i>OK201Y>UAS-GFP</i> (shown in green). (A, A’) Wild-type mushroom bodies are shown. α, β and γ lobes can be distinguished by their distinct projection patterns and fluorescent labeling. <i>OK201Y >UAS-GFP</i> is expressed mainly in the γ lobes and weakly in the α and β lobes; anti-FasII labels the α and β lobes strongly and γ lobes weakly. (B-E) Abnormal MB morphology seen in <i>Nipped-B</i> heterozygotes. (B) short α lobe and β lobe missing. (C) thinner α lobe and β lobes. (D) β lobe missing. (E) kinky α lobe. (F) A higher percentage of <i>Nipped-B</i> heterozygous MBs are morphologically abnormal compared to controls. The differences are statistically significant. Numbers of abnormal MBs versus numbers of normal MBs counted in each genotype are labeled next to the columns. Statistical significance was determined through Fisher’s exact tests. *, p<0.05; **, p<0.01.