Neural activity in mushroom body αβ neurons is required for normal egg-laying decisions.

<p>(A) Preferential expression of <i>R16A06-GAL4</i> in mushroom body γ neurons (green). The brain was immunostained with DLG antibody (red). The scale bar represents 50 μm. Genotype was as follow: <i>UAS-mCD8</i>::<i>GFP/+; R16A06-GAL4/UAS-mCD8</i>::<i>GFP</i>. (B) Preferential expression of <i>VT30604-GAL4</i> in mushroom body α´β´ neurons (green). The brain was immunostained with DLG antibody (red). The scale bar represents 50 μm. Genotype was as follow: <i>UAS-mCD8</i>::<i>GFP/+; VT30604-GAL4/UAS-mCD8</i>::<i>GFP</i>. (C) Preferential expression of <i>VT49246-GAL4</i> in mushroom body αβ neurons (green). The brain was immunostained with DLG antibody (red). The scale bar represents 50 μm. Genotype was as follow: <i>UAS-mCD8</i>::<i>GFP/+; VT49246-GAL4/UAS-mCD8</i>::<i>GFP</i>. (D) Effects of acute silencing of neuronal activity in different mushroom body neuron subsets on egg-laying decisions. The temperature shift protocols are shown schematically above each graph. Each value represents mean ± SEM (n = 10–25, ***P < 0.001. n.s., not statistically significant). Genotypes were as follows: (1) <i>tubP-GAL80</i>^<i>ts</i><i>/+; +/UAS-Kir2</i>.<i>1</i>, (2) <i>+/+; R16A06-GAL4/+</i>, (3) <i>+/+; VT30604-GAL4/+</i>, (4) <i>+/+; VT49246-GAL4/+</i>, (5) <i>tubP-GAL80</i>^<i>ts</i><i>/+;R16A06-GAL4/UAS-Kir2</i>.<i>1</i>, (6) <i>tubP-GAL80</i>^<i>ts</i><i>/+;VT30604-GAL4/UAS-Kir2</i>.<i>1</i>, and (7) <i>tubP-GAL80</i>^<i>ts</i><i>/+;VT49246-GAL4/UAS-Kir2</i>.<i>1</i>.