CG4395-Gal4 expression in male brains.

<p>(A). Z-stack of anti-β-Gal staining of isolated male brains of <i>4395-Gal4/UAS-lacZ^nls</i>. (B,C). Brains of <i>4395-Gal4/UAS-lacZ^nls</i> males were double-stained with anti-β-Gal (red) and anti-Dco (green). Dco is present in the cytoplasm of the cell bodies of the mushroom bodies and projections of the mushroom bodies <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0028269#pone.0028269-McGuire1" target="_blank">[24]</a>. Optical sections are shown. Anti-β-Gal staining is seen in a number of mushroom body cell bodies (arrowhead), but absent from others (arrow). A magnification of (B) is shown in (C). (D–I). Brains of <i>4395-Gal4/UAS-mcD8::GFP</i> males were double-stained with anti-GFP (green) and nc82 to visualize neurons (magenta). Projections can be seen in the mushroom bodies (D, Z-stack), the central complex (E, Z-stack) and a subset of glomeruli in the antennal lobes (F, selected optical section). Staining was observed in glomeruli DA1, VA1v, VA1d, VA6, DM3 and DL3 (F). (G–I) To visualize mb projections, double staining was performed with anti-GFP and anti-Dco (G, merged), the single channels are shown in (H) and (I); Z stacks are shown. Abbreviations: (nls) nuclear localization signal; (mcD8::GFP) membrane bound GFP that visualizes neuronal extensions. (mb) mushroom bodies; (ca) calyx of the mushroom bodies; α, β γ: α, β, γ lobes of the mushroom bodies.