<i>hrp48</i> downregulation results in MB lobe projection defects.

<p>(A-C) Mushroom Body (MB) lobes of control (A), <i>hrp48</i>^{<i>k10413</i>}/<i>hrp48</i>^{<i>k16203</i>} (B), or <i>hrp48</i>^{<i>k10413</i>}/<i>hrp48</i>^<i>02647</i> (C) adult brains stained with anti-FasciclinII (FasII) antibodies. FasII is strongly expressed in αβ neurons, weakly in γ neurons. (D-F) MB lobes of control (D) or <i>hrp48</i>-RNAi (E,F) adult brains expressing mCD8-GFP (green), and stained with anti-FasII antibodies (red). Representative examples of the asymmetric and truncated categories used for quantification are shown. Precise genotypes: UAS-mCD8-GFP/+;;OK107-Gal4/+ (D); UAS-mCD8-GFP/UAS-RNAi-<i>hrp48</i>^{<i>#101555</i>};;OK107-Gal4/+ (E,F); UAS-mCD8-GFP/+;UAS-RNAi-<i>hrp48</i>^{<i>#16041</i>}<i>/+</i>;OK107-Gal4/+ (G). Scale bar in A-F: 20μm. (G) Percentages of MBs exhibiting symmetric projections, asymmetric projections or truncated lobes (either α or β) in controls (<i>w</i>), <i>hrp48</i> transheterozygous combinations, and two independent RNAi lines (#16041 and #101555). Two controls were used for the rescue assay: UAS-mCD8-GFP/UAS-RNAi-<i>hrp48</i>^{<i>#101555</i>};UAS-GFP/+;OK107-Gal4/+ flies as a control of Gal4 titration, and UAS-mCD8-GFP/+;UAS-Flag-Hrp48/+;OK107-Gal4/+ flies to ensure that Hrp48 overexpression did not induce axon projection defects. Numbers represent numbers of scored hemispheres. Statistical comparison to the UAS-GFP control: ***, p<0.001 (χ² test).