Effects of CD2 on ouabain-induced signal transduction Cell lines were treated with indicated concentrations of ouabain for 10 minutes.

<p>Cell lysates were collected and analyzed for A) pY418 Src and total cellular Src (c-Src), B) phospho-ERK1/2 and total ERK1/2 C) phospho- Akt and total Akt. Representative Western blots are shown and quantitative data are mean± SEM of at least three independent experiments. * p<0.05, compared with 0nM control, #, p<0.05, compared with different cell line. D) Five hundred μg of total cell lysate was immunoprecipitated with 10μg of anti-Src antibody and analyzed for co-precipitated Na/K ATPase α1. A representative western blot is shown. Values are mean ± SEM from at least three independent experiments. * p<0.05 compared with control.