Additional file 2: Figure S2. of Amyloid β-protein oligomers upregulate the β-secretase, BACE1, through a post-translational mechanism involving its altered subcellular distribution in neurons

Effects of chloroquine on BACE1 levels and immunoreactivities in primary neurons. (A) Primary neurons were treated with or without 25 μM chloroquine (CQ) for 1 day, followed by Western blot analysis with anti-BACE1. Relative levels of BACE1 were quantified and graphed. Data represent means ± SEM of three samples from two separate experiments. *p < 0.05, compared with control. (B) Primary cortical neurons treated with or without 25 μM CQ for 1 day were immunostained with anti-BACE1. Scale bar = 20 μm. (C) Fluorescence intensities of BACE1 in soma and neurites in (B) were separately quantified as described in Methods, and the relative levels depicted on a graph. (n = 24, ***p < 0.001). (PDF 1277 kb