Additional file 2: Figure S2. of VPS35-deficiency results in an impaired AMPA receptor trafficking and decreased dendritic spine maturation

VPS35 interaction with GluA1 and GluA2. (A) Co-immunostaining analysis of endogenous VPS35 with GFP-GluA1/GFP-GluA2 in cultured hippocampal neuron expressed GFP-GluA1/GFP-GluA2. Scale bars, 5 μm. (B) Quantification of colocalization index (overlapping signal over total GFP) in A. Data were shown as mean ± SEM; n = 30 neurons from 3 independent experiments; *p < 0.05. (C) Coimmunoprecipitation analysis of exogenously expressed flag-VPS35 and GFP-GluA1. HEK293 cells transfected with indicated plamids were lysed and subjected to co-immunoprecipitation assays. The resulting lysates were loaded onto SDS-PAGE gels and immunoblotted with indicated antibodies. (TIFF 14153 kb