Additional file 2: of JNK-mediated microglial DICER degradation potentiates inflammatory responses to induce dopaminergic neuron loss

Related to Fig. 2 (a) Representative immunoblots of total lysates of BV2 cells treated with 100 μM MPP+ for the indicated times with the antibody against α-spectrin. (b) Representative immunoblots of total lysates of primary cultured neurons treated with 50 μM glutamate (Glu) or/and calpeptin (CAL) or MDL28170 (MDL) for 4 h with the antibody against α-spectrin. (c) Representative immunoblots of total lysates of BV2 cells treated with MPP+ for the indicated times with the antibody against caspase-3 and cleaved caspase-3. (d) Statistics of DICER in Fig. 2e. (e) Upper, representative immunoblots of total lysates from BV2 cells treated with 100 μM MPP+ with/without LAC (1 μM) (e) or MG115 (1 μM) (f) for 12 h and detected with the indicated antibodies. Lower: statistics. (g) Representative immunoblots of immunoprecipitates for total lysates of BV2 cells treated with MG115 with or without MPP+ and then probed with anti-ubiquitin antibody. Data are shown as mean + SEM. *p < 0.05, **p < 0.01, ***p < 0.001. α-tubulin (α-Tub), a loading control. kDa, kilodalton. (PNG 512 kb